大孔吸附树脂对喜树叶中喜树碱的纯化工艺研究
Science & Technology in Chemical Industry（2015）
Abstract
研究通过静态吸附/解吸实验对大孔吸附树脂进行筛选,优选AB-8大孔吸附树脂作为层析柱填料,并对其进行喜树碱纯化工艺研究;研究表明AB-8树脂对喜树碱的静态吸附率为95.31％;体积分数95％的乙醇静态解吸率为92.4％;最佳吸附条件为:上样液质量浓度为0.175 mg/mL,上样液不调pH值,吸附流速为2BV/h,平衡吸附5h;最佳洗脱条件:体积分数95％乙醇,洗脱流速1BV/h,洗脱体积为8BV.在该工艺条件下,洗脱物中喜树碱质量分数为7.43％,洗脱率为83.1％.
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