纸层析-酶标仪法测定发酵液中γ-氨基丁酸
Food and Fermentation Industries（2019）
Abstract
建立纸层析-酶标仪法对微孔板发酵液中γ-氨基丁酸产量进行测定.对影响纸层析-酶标仪法测定结果的重要因素进行优化,并建立相应的分光光度法.优化后层析液中显色剂-茚三酮的浓度为8 g/L,展开剂-正丁醇、冰醋酸与水3者体积比为2∶1∶1;洗脱液中乙醇的体积分数为75％,乙醇与质量浓度为6 g/LCuSO4·5H2O的体积比为39∶1;γ-氨基丁酸质量浓度在1～7 g/L之间,吸光度与样品浓度线性关系良好(R2=0.999 0),平均回收率为98.248 5％,重复性RSD为2.625 5％.纸层析-酶标仪法能快速、准确地测定微孔板发酵液中γ-氨基丁酸产量,为后续高通量选育γ-氨基丁酸高产菌株奠定了基础.
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