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产葡萄糖氧化酶菌株的诱变筛选及遗传稳定性研究

Guoqin XIN,Peipei DONG,Xiangyan WANG,Ying ZHAO,Xiuxia LIU,Haiyan XU,Wei GU

China Brewing（2016）

Cited 2|Views4
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Abstract
采用平板显色初筛、Underbofler滴定法复筛及紫外-硫酸二乙酯复合诱变的方法,从临沂果园土样中得到一株产葡萄糖氧化酶(GOD)活性较好的菌株,命名为Bla-6,其产GOD酶活为(10.50±0.25) U/mL,与原始菌株LPA-4产GOD酶活(6.75 U/mL)相比提高了55.57％.结合5.8S rDNA-ITS区序列系统进化、菌落形态、生长特性、菌体形态等确定其分类地位.结果表明,所得菌株Bla-6被鉴定为黑曲霉(Aspergillus niger),经8代传代培养后,GOD酶活维持在10.5 U/mL左右,该菌株遗传稳定性较好.
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