L-异亮氨酸高产菌种的选育研究
China Brewing(2014)
Abstract
以谷氨酸棒状杆菌(C orynebacterium glutam icum)为出发菌株,利用低能氮离子束注入对其进行多次诱变,结合异亮氨酸氧肟酸盐(IleH x)等氨基酸结构类似物定向筛选,得到一株稳定的高产L-异亮氨酸菌株:摇瓶培养72 h,产酸可达21~22 g/L,在50 L发酵罐上发酵51 h,产酸可达32 g/L。
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