响应面法优化蜡样芽孢杆菌产类细菌素培养基
Science and Technology of Food Industry(2016)
Abstract
采用响应面分析法对产类细菌素的蜡样芽孢杆菌XH25(Bacillus cereus XH25)培养基进行优化,以金黄色葡萄球菌为指示菌,抑菌圈直径作为响应值.利用Plackett-Burman设计筛选出影响抑菌圈直径的显著因素:pH、可溶性淀粉和(NH4)2SO4.通过最陡爬坡实验逼近最大抑菌圈直径区域.采用中心组合设计法及响应面分析确定:pH6.15、可溶性淀粉15.32 g/L和(NH4)2SO46.02 g/L.从节约成本考虑,其他不显著因素均保持低水平浓度:酵母粉5.00 g/L、蔗糖5.00 g/L、豆粕粉5.00 g/L、MgSO40.20 g/L和K2 HPO42.00 g/L.在该条件下,抑菌圈直径预测值为12.76 mm,实验测定值为12.92 mm,实验结果与模型预测值吻合,说明所建模型是切实可行的,优化后抑菌活力比优化前(10.62 mm)提高了21.66%,为Bacillus cereus XH25大规模发酵产类细菌素奠定一定基础.
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