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高效液相色谱法测定小麦粉中偶氮甲酰胺

Li ZHAO,Ming-yuan SHAO,Ya ZHOU,Ming-yan LIU

China Food Additives（2018）

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Abstract
建立了小麦粉中偶氮甲酰胺含量的高效液相色谱方法,并采用液质联用法进行确证.该方法经N,N-二甲基甲酰胺溶液提取,用三苯基膦衍生,以CapcellpakC 18 UG 120 (4.6mm×250mm,5μm)柱分离,流动性为甲醇和水溶液梯度洗脱,高效液相色谱仪-二极管阵列检测器测定,外标法定量.该方法检出限为1mg/kg,定量限为3mg/kg,线性范围1～ 100μg/mL,加标回收率99.8％～ 106.3％,相对标准偏差为1.12％ ～ 5.09％.
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