响应面法优化大孔树脂纯化杜仲绿原酸工艺研究
Food Research and Development（2018）
Abstract
以杜仲绿原酸提取物为原料,考察6种大孔树脂对杜仲提取物中绿原酸的静态吸附与洗脱性能,结合静态吸附动力学结果,确定采用DM-130型大孔树脂纯化提取物中绿原酸.采取响应面法进行动态吸附-洗脱试验,得出最佳纯化工艺为:5.5 mg/mL杜仲提取液,以2.5 BV/h流速,上样至DM-130型大孔树脂中吸附,随后采用7.5 BV的55 %乙醇溶液,以2.0 BV/h流速洗脱.通过紫外-可见分光光度法定量分析表明,提取物中绿原酸含量由纯化前134.5 mg/g提高至纯化后341.2 mg/g,表明响应面法优化所得大孔树脂纯化工艺方便可行.
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