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豆豉中产甲基萘醌菌株分离、筛选及其鉴定

Food Research and Development(2018)

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Abstract
通过平板初筛,从江西产发酵型豆豉中分离得到29株芽孢杆菌.经过高效液相色谱法(high performance liquid chromatography,HPLC)初步定性定量检测,得16株产甲基萘醌菌株,其中DC-1为甲基萘醌(menaquinone,MK)产量较高菌株,确定为目标菌株.通过液质连用(liquid chromatograph-mass spectrometer,LC-MS)对DC-1菌株代谢产物分析,确定产物为MK-7.该菌株经形态学,生理生化试验,分子生物学试验和16S rDNA基因序列比对结果分析,鉴定为枯草芽孢杆菌Bacillus subtilis DC-1.通过该菌株初步发酵检测结果表明,发酵培时间为4 d时,Bacillus subtilis DC-1代谢物MK-7产量最高,可达到0.416 mg/L,为生物发酵生产MK-7提供了有利的微生物资源.
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