固相萃取-液质联用测定火锅底料中5种生物碱

Food Research and Development（2016）

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Abstract

建立双柱串联固相萃取-高效液相色谱-串联质谱法(HPLC-MS/MS)测定火锅底料中罂粟碱、吗啡、那可丁、可待因和蒂巴因5种生物碱成分的含量.样品用酸化乙腈提取,PSA和PXC固相萃取柱串联净化,经Hillic色谱柱分离,以电喷雾离子源(ESI)在正离子多反应监测(MRM)模式下进行测定,内标法定量.在优化的试验条件下,罂粟碱、吗啡、那可丁、可待因、蒂巴因的检出限分别为0.033、1.5、0.066、1.2、0.15μg/kg.5种生物碱类药物在3个加标水平下的回收率范围为96.0%~112.5%,RSD为2.0%~5.7%.该方法操作简便、准确、灵敏度高,适用于火锅底料中罂粟碱、吗啡、那可丁、可待因和蒂巴因含量的测定.

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