酶解海产低值鱼制备抗氧化肽的研究

The Food Industry(2017)

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Abstract
酶解海产低值鱼制备抗氧化肽,分别研究底物浓度、pH、温度、酶浓度及时间对酶解产物清除DPPH·的影响.在单因素试验基础上,通过正交试验对酶解制备抗氧化肽工艺进行优化,确定最佳工艺为:中性蛋白酶添加量3%,底物浓度6%,温度55℃,反应时间3h,反应pH 7.0.在最佳条件下,所得多肽的质量浓度为164.64 μg/mL,DPPH·清除率为68.633%.
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