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甘蔗CP84-1198×ROC22杂交群体抗褐锈病Bru1基因分子检测

Xing-long CHEN,Jin-fang XIE, Bin WU,Xiao-min FENG,Yong-hong HUANG,Xiao-ning GAO,Rui LIU,Jia-yun WU,Jun-jia CHEN,Yong-wen QI

Sugarcane and Canesugar（2020）

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Abstract
为了更好地改良ROC22的农艺性状,本研究以ROC22为父本,CP84-1198为母本,构建了CP84-1198×ROC22杂交群体(1100个株系).从杂交后代中挑取优良个体材料,以褐锈病抗性为指标,随机挑取280个株系苗期的叶片,运用CTAB方法提取甘蔗基因组DNA,以R12H16为标记用普通PCR技术对该群体各个株系进行锈病抗性基因Bru1检测,结果发现该杂交群体中156个(55.71％)株系检测到了该基因,含有与不合有抗锈病基因Bru1符合理论比1∶1,研究结果表明ROC22含有单个抗锈病Bru1等位基因,CP84-1198不合有抗锈病Bru1等位基因.以期能为深入开展甘蔗群体抗褐锈病育种、选育和推广优良抗性品种(系)提供材料基础.
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