芦笋组培快繁技术研究
LiaoNing Forestry Science and Technology(2020)
Abstract
为建立芦笋组培快繁体系,以芦笋种子为试验材料,以MS培养基为基本培养基,附加2%蔗糖和0.7%琼脂,添加不同质量浓度的激素进行愈伤组织诱导、丛生芽诱导,并进行生根及移栽试验.结果表明:愈伤组织诱导最适培养基为MS+6-BA 1.0 mg·L-1,诱导率95.34%;丛生芽诱导最适培养基为MS+6-BA 0.5 mg·L-1+NAA 0.2 mg·L-1,增殖系数9.43;最适生根培养基为1/2 MS+IBA1.0 mg·L-1.试管苗经炼苗后移栽至泥炭土:河沙=5:1的基质中,成活率82%.
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