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利用高速逆流色谱技术制备灵芝酮三醇

YUE Yawen,TIAN Zhen,ZHANG Jingsong,WANG Chenguang,HAN Wei,TANG Qingjiu,FENG Na,ZHOU Shuai

Acta Edulis Fungi（2020）

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Abstract
以沪农灵芝(Ganoderma lucidum)1号子实体为原料,经过87％乙醇提取、D101大孔树脂富集、乙醇梯度洗脱获得弱极性三萜组分.采用高速逆流色谱技术,以正己烷∶乙酸乙酯∶甲醇∶水(V∶V∶V∶V=5∶6∶5∶5)为溶剂体系,对该三萜组分中灵芝酮三醇的制备工艺进行研究,在转速900 r·min-1、流速3 mL· min-1条件下,一次上样400 mg灵芝弱极性三萜组分,可得到纯度为91.6％的灵芝酮三醇26.8 mg.
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