窖泥中产丁酸菌的筛选、鉴定及生长性能研究
Liquor-Making Science & Technology(2017)
Abstract
通过对川南某酒厂的老窖泥富集培养、分离纯化,经丁酸发酵实验,筛选出1株高产丁酸的梭菌.经16Sr DNA鉴定,该菌为丁酸梭状芽孢杆菌(Clostridium butyricum),命名为丁酸菌YD-4.经对该菌株生长性能条件研究,其最适生长温度为37℃,最适pH值为7.0,最适接种量为2%,菌体浓度达1.64×108个/mL,产丁酸量可达到3.44 g/L.
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