乳茄组培快繁技术

Anhui Agricultural Science Bulletin(2020)

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Abstract
以乳茄嫩枝为外植体,研究了不同消毒方法和不同激素对乳茄诱导、增殖及生根等的影响.结果表明,最适合乳茄外植体的消毒方法为70%的酒精消毒10s,0.1%的升汞消毒6min;最佳芽诱导培养基为MS+6-BA2.0mg·L-1+NAA0.05mg·L-1;最佳继代增殖培养基为MS+BA1.0mg·L-1+NAA0.05mg·L-1+GA30.1mg·L-1+蔗糖30g·L-1,增殖系数为4.35;最佳生根培养基为1/2MS+IBA0.5mg·L-1+ABT10.3mg·L-1,生根率达93.5%以上;组培苗移栽适宜基质为泥炭土:珍珠岩=5:1,成活率达96.7%.
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