水稻根际促生菌的筛选鉴定及促生能力分析
Journal of Agricultural Resources and Environment(2018)
Abstract
植物根际促生菌(Plant growth-promoting rhizobacteria,PGPR)可分泌植物生长激素,促进土壤养分循环,是生物肥料重要的种质资源.本文从水稻根际土壤分离纯化根际促生菌,进行菌株鉴定,测定其促生能力.经过16S rDNA测序比对,筛选得到解磷菌4株(Bacillus pumilus LZP02,Bacillus aryabhattai LZP08,Staphylococcus epidermidis LZP10,Bacillus ginsengisoli LZP05),溶磷菌3株(Bacillus megaterium LZP03,Bacillus oryzaecorticis LZP04,Bacillus ginsengisoli LZP07),解钾菌3株(Bacillus aryabhattai LZP01, Bacillus subtilis LZP06,Bacillus licheniformis LZP09).养分转化能力测试结果表明,Bacillus aryabhattai LZP01和 Bacillus subtilis LZP06解钾能力较好;Bacillus pumilus LZP02和Bacillus huizhouensis LZP05解磷能力较强;Bacillus megaterium LZP03和Bacillus ginsengisoli LZP07溶磷能力较好.对养分转化能力较强的菌株进行激素分泌能力测定,结果表明6种菌株均能产生生长素、赤霉素,均具有合成铁载体的能力.综合分析菌株养分转化与激素分泌能力发现,Bacillus megaterium LZP03、Bacillus huizhouensis LZP05和Bacillus subtilis LZP06促生能力较强,具有较强的开发利用潜力.研究成果为水稻微生物肥料的开发与生产提供了理论和技术支持.
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