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黑脉羊肚菌固体培养基的制备及其培养条件优化

Bao-shun ZHANG, Meng-ting YE,Qing LIU,Jian-kang WANG,Nan-xuan LUO,Chang-qing YANG,Yu-rong ZHU,Zhu-bo LI,Yuan-xi LIU

Journal of Southwest China Normal University(Natural Science Edition)（2020）

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Abstract
以黑脉羊肚菌为对象,研究了不同固体培养基对菌种生长的影响.通过以菌丝生长速度、密度和菌核为考察指标,研究在不同碳源、氮源、无机盐及pH条件下羊肚菌菌丝体的生长状况,从而优化培养基的制备工艺.结果表明:黑脉羊肚菌生长的最适条件为2％ 的葡萄糖碳源,2 g/L的蛋白胨氮源,MgSO4无机盐,pH值为6.0.该实验为今后羊肚菌的栽培奠定了坚实的基础.
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