Primary tumor cell isolation and culture method

A primary tumor cell isolation and culture method comprises the following steps: removing necrotic and non-tumor tissue around tumor tissue of a tumor specimen; cutting and digesting the tumor tissue,and filtering the obtained tissue after digestion through a sieve, conducting centrifugation and PBS resuspension, conducting centrifugation again, and discarding the supernatant to obtain isolated tissue cells; resuspending the isolated tissue cells in a primary cell serum-free medium to obtain a resuspension, adjusting cell density, and inoculating a primary cell ultra-low adhesion culture dish; every 48 hours, conducting centrifugation and cell collection, discarding the original primary cell serum-free medium supernatant, and conducting resuspension liquid replacing with the primary cellserum-free medium; continuing culturing the cells in the primary cell ultra-low adhesion culture dish for a week or more; and then culturing the cells by a conventional cell culture method to obtain high-purity primary tumor cells. The cells prepared by the method have high acquisition rate and high degree of purification; and the method has no higher requirements on existing experimental equipment, and is suitable for wide application and promotion in the field of tumor research.