Express Arabidopsis Cryptochrome in Sf9 Insect Cells Using the Baculovirus Expression System.

PLANT PHOTOMORPHOGENESIS: Methods and Protocols（2021）

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摘要

The Bac-to-Bac Baculovirus Expression System provides a rapid and efficient method to generate recombinant cryptochrome (CRY) proteins with chromophore flavin (FAD), which showed blue light response in vitro.

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关键词

Arabidopsis,Baculovirus expression system,Blue light response,Cryptochrome,FAD,Sf9 insect cell

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