Primer group for identifying avian nephritis virus and chicken infectivity anemia virus and application thereof

The invention discloses a primer group for identifying avian nephritis virus and chicken infectivity anemia virus. The primer group comprises a primer pair I and a primer pair II, wherein the primer pair I is composed of primers ANV-F and ANV-R, and the primer pair II is composed of primers CIAV-F and CIAV-R; the primers ANV-F, ANV-R, CIAV-F and CIAV-R have base sequences from SEQ. ID. NO.1 to SEQ. ID. NO.4 in the sequence table respectively. Therefore, the inventor also establishes a corresponding detection and identification method and develops a corresponding test kit. The duplex PCR established by the invention can be used for rapidly detecting mixed infection of avian nephritis virus and chicken infectivity anemia virus, is suitable for large-batch detection, can save cost and time, can reduce pollution, and has very high practical value.