Bai yang dian virus and duck plague virus double fluorescent quantitation RT-PCR detection reagent kit

The invention discloses a bai yang dian virus and duck plague virus double fluorescent quantitation RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection reagent kit which comprises a primer group and a probe group, wherein the primer group comprises a primer 1, a primer 2, a primer 3 and a primer 4; the primer 1, the primer 2, the primer 3 and the primer 4 have base sequences of SEQ. ID. No. (Sequence Identifier Number) 1, SEQ. ID. No. 2, SEQ. ID. No. 3 and SEQ. ID. No. 4 respectively; the probe group comprises a probe A and a probe B; and the probe A and the probe B have base sequences of SEQ. ID. No. 5 and SEQ. ID. No. 6 respectively. An experiment proves that the reagent kit has the advantages of short detection time, high detection sensibility and high specificity, can detect and identify a bai yang dian virus and a duck plague virus simultaneously, and can quantify the content of corresponding pathogeny of the bai yang dian virus and the duck plague virus, so that the reagent kit can be used for evaluation of curative effects of bai yang dian virus and/or duck plague virus vaccine and drug, and for research of pathogenesis and the like, and has important significance in prevention and cure of the bai yang dian virus and the duck plague virus.