The Effect Of Q-Switched 1064-Nm Nd: Yag Laser On Skin Barrier And Collagen Synthesis Via Mir-663a To Regulate Tgf Beta 1/Smad3/P38mapk Pathway

Background: Our previous research found that Q-switched 1064-nm Nd: YAG laser (1064-QSNYL) induces skin collagen synthesis by activating TGF beta 1/Smad3/ p38MAPKs pathway. Moreover, a lot of studies shown that MicroRNAs (miRNAs) contribute to regulate collagen synthesis and skin barrier. Therefore, we intend to explore the mechanism of 1064-QSNYL on collagen synthesis and skin barrier through miRNAs.Methods: We predicted the upstream miRNAs of TGF beta 1 by bioinformatics databases, and verified them through dual-luciferase reporter genes and Western blotting. The expression of collagen, skin barrier-related protein K10 and filaggrin, TIMP-1, and MMP-2 were detected by RT-qPCR and Western blotting, respectively. Moreover, we detected moisture content, elasticity value, TEWL value, SOD vitality, and hydroxyproline content to evaluate skin barrier of mice. H&E staining to observe the change of dermis thickness and inflammation and infiltration of mice skin.Results: The results shown that TGRA was target gene of miR-663a. Moreover, we found that 1064-QSNYL activated TGF beta 1/smad3/p38MAPK pathway by downregulating the expression of miR-663a in HaCaT, HDF cells, and mice, thereby promoting expression of Collagen I, Collagen IV, TIMP-1, Kb, and filaggrin and inhibiting MMP-2. Furthermore, 1064-QSNYL contributed to moisture content, elasticity, SOD vitality, and hydroxyproline content via miR-663a to activate TGF beta 1/smad3/ p38MAPK pathway.Conclusions: In summary, this study found for the first time that 1064-QSNYL contributed to collagen synthesis and skin repair via miR-663a to regulate TGF beta 1/smad3/ p38MAPK pathway, thereby achieving skin rejuvenation.