Effect of MCP-1 and CCR2 Genes Polymorphism on Development of Hepatocellular Carcinoma in HCV- Infected Patients in Sohag Governorate, Egypt

Background: HCC is the most common primary tumor of the liver .It is the fifth common cancer in men and the eighth common in women and is the second leading cause of cancer-related death in the world. The MCP-1 is a chemokine and a potent chemotactic factor for monocytes. MCP-1 expression in tumor cells is significantly linked to the extent of tumor-associated macrophage infiltration. Objectives: to detect the effect of MCP-1 and CCR2 Genes Polymorphism in development of HCC in HCV- related liver cirrhosis patients. Methodology: MCP-1-2518 A/G and CCR2 (V64Ile) genes polymorphism was assessed by real time PCR in 35 HCC patients and 30 HCV related LC. Serum MCP-1 was measured by ELISA. Results: For MCP-1 -2518 A G gene, HCC patients had a higher frequency of AG and GG genotypes than of AA genotype compared to other groups. For CCR2 (V64Ile), HCC patients had a higher frequency of GA and AA genotypes than GG genotype compared to other groups. Conclusion: There is a significant association between CCR2 (V64Ile) polymorphism, high serum MCP-1 level and HCC development in HCV- related liver cirrhosis patients.