Sarcosine detection method based on metal organic framework material

The invention relates to a sarcosine detection method based on a metal organic framework material. The sarcosine detection method is characterized in that sarcosine oxidase and catalase are wrapped bymeans of the metal organic framework material, so that the pair of cascade enzymes is limited in a smaller space, and the content of sarcosine is determined by adopting a chemiluminescence method after a cascade reaction. The sarcosine detection method adopts ZIF-8 to coat SOX and HRP; ZIF-8 has a large specific surface area and porous channels, so that a reaction substrate in a solution can penetrate through the channels to react with SOX and HRP, enrichment of the substrate around the enzymes is facilitated, and the local substrate concentration is increased; meanwhile, due to wrapping of ZIF-8, SOX and HRP are limited within the range of hundreds of nanometers, the distance between SOX and HRP is regulated and controlled, hydrogen peroxide generated by catalyzing sarcosine by means ofSOX can be effectively transmitted to HRP for a chromogenic reaction, and the reaction rate of the reaction cascade enzymes is increased.