An overview of open tubular liquid chromatography with a focus on the coupling with mass spectrometry for the analysis of small molecules

•OT-LC features high peak capacity, great separation power and short analysis times•OT-LC chemistry and fabrication technologies offer new separation opportunities•OT-LC- ESI-MS provides enhanced sensitivity and reduced matrix effects•OT-LC-EI-MS offers superior compatibility and unparalleled detection capabilities•OT-LC-MS is becoming a powerful tool in the analysis of bio- and small molecules