Ultrafiltration combing with phospholipid affinity-based isolation for metabolomic profiling of urinary extracellular vesicles
Journal of Chromatography A（2021）
Abstract
•Ultrafiltration Combining with phospholipid affinity for isolating urinary EVs.•This method comparable to ultracentrifugation regarding EV quality.•EV untargeted/lipid metabolomic profiling performed.•The proposed method works when ultracentrifugation is not accessible.
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Key words
Extracellular vesicle,Phospholipid affinity,Ultracentrifugation,Metabolomic profiling,Lipidomic profiling
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