Development of in-house ELISAs as an alternative method for the serodiagnosis of leptospirosis

•Confirmatory laboratory diagnosis of leptospirosis is a major challenge.•In-house ELISAs were established to detect anti-leptospiral antibodies.•Factors responsible for the manifestation of severe leptospirosis forms are unclear.•High anti-leptospiral IgG was observed in severe leptospirosis patients compared to mild leptospirosis patients.•Anti-leptospiral IgG could be used as a biomarker to predict disease severity.