Clostridium Vitabionis Sp. Nov., Isolated From The Large Intestine Of A Mini-Pig

An obligately anaerobic, Gram- stain- negative, spore-forming, short rod-shaped bacterium, designated strain YH-T4B42(T), was isolated from the large intestine of a mini- pig. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belongs to the genus Clostridium and is most closely related to Clostridium aminophilum KCTC 5424(T), Clostridium sym-biosum KCTC 15329(T) and Clostridium butyricum KCTC 1871(T), with 95.5, 92.4 and 83.0 % sequence similarity, respectively. The average nucleotide identity values for strain YH- T4B42(T) and the closest related strains were lower than 72 %. The G+C content of the isolate was 55.8 mol%. The cell- wall peptidoglycan was A1 gamma type and contained meso-diaminopimelic acid. The pre-dominant fatty acids were C-16:0, C(18:1)cis 9, C-14:0 and C-18:0. The major end products of glucose fermentation were lactate, formate and acetate, with a minor amount of butyrate. Based on its phenotypic, phylogenetic and chemotaxonomic properties, a novel species, Clostridium vitabionis sp. nov., is proposed for strain YH-T4B42(T) (=KCTC 25105(T)=NBRC 114767(T)).