Mapping Cell Viability Quantitatively and Independently From Cell Density in 3D Gels Noninvasively

Brian Archer,Julia Mack, Sara Acosta, Russell Nakasone,Fadi Dahoud,Khalid Youssef, Abraham Goldstein, Amichai Goldsman,Mathias Clemens Held,Martin Wiese,Bernhard Bluemich,Matthias Wessling,Meike Emondts,Juergen Klankermayer,Luisa Iruela-Arispe,Louis Bouchard

IEEE Transactions on Biomedical Engineering（2021）

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摘要

Objective: In biomanufacturing there is a need for quantitative methods to map cell viability and density inside 3D bioreactors to assess health and proliferation over time. Recently, noninvasive MRI readouts of cell density have been achieved. However, the ratio of live to dead cells was not varied. Herein we present an approach for measuring the viability of cells embedded in a ...

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关键词

Magnetic resonance imaging,Nuclear magnetic resonance,Statistics,Sociology,Electron tubes,Three-dimensional displays,Density measurement

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