Metabolizable amino acids and energy requirements of Nellore and crossbred Angus x Nellore bulls fed rations of different crude protein concentrations

Growth rate of cattle depends on their genetic makeup and nutrient intake. Moreover, increased growth rate may lead to increased amino acid (AA) requirements. Therefore, we evaluated the AA content of the empty body and estimated the net M and energy requirements of purebred and crossbred beef bulls fed rations of different dietary CP concentrations. We performed a comparative slaughter experiment with 24 Nellore and 24 Angus x Nellore (A x N) bulls (8 months; initial shrunk BW: Nellore = 208.0 +/- 12.78 kg; A x N = 221.9 +/- 14.16 kg). Eight bulls (four Nellore and four A x N) were designated as the reference group, eight bulls (four Nellore and four A x N) were fed to maintenance level and 32 bulls (16 Nellore and 16 A x N) were fed ad libitum. The 32 bulls fed ad libitum were distributed using a completely randomized design in a 2 x 3 factorial scheme with two genetic groups (Nellore or A x N) and three dietary CP contents (100, 120 or 140 g CP/kg DM), being four groups with rive bulls and two groups with six bulls. The experimental period lasted for 224 days. There were no interactions (P >= 0.056) between the dietary CP contents and genetic groups for any of the response variables. The dietary CP contents did not affect (P >=. 0.062) the AA content in the empty body (g/kg empty BW IEBWD, with exception for Tryptophan (P = 0.027, linear effect). The dietary CP contents did not affect (P >= 0.051) AA content in the empty body (g/100 g of CP), with exception for Alanine (P = 0.013) that responded quadratically to dietary CP increase. The equations to estimate the net Lysine (Lys) and Methionine (Met) requirements (g/100 g of CP) were: Lys = 5.1 x EBW0.0594 and Met = 1.7 x EBW0.0255. Metabolizable Lys and Met to metabolizable energy (ME) ratios decreased as bulls EBW increased. Also, the metabolizable protein to ME ratio decreased as bulls EBW increased. In conclusion, the present study provides useful information regarding net and metabolizable requirements of AA of purebred and crossbred beef bulls. In the future, after the validation of the equations, these results can be used to calculate the AA requirements for growth of purebred and crossbred beef bulls. Nevertheless, it is important to highlight that the small sample size was one limitation of this present experiment. (C) 2020 Published by Elsevier Inc. on behalf of The Animal Consortium.