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Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA

Ute Eberle,Clara Wimmer,Ingrid Huber,Antonie Neubauer-Juric, Giuseppe Valenza,Nikolaus Ackermann,Andreas Sing,Armin Baiker

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES（2021）

Cited 16|Views6
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Abstract
To face the COVID-19 pandemic, the need for fast and reliable diagnostic assays for the detection of SARS-CoV-2 is immense. We describe our laboratory experiences evaluating nine commercially available real-time RT-PCR assays. We found that assays differed considerably in performance and validation before routine use is mandatory.
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Key words
SARS-CoV-2, Molecular testing, Laboratory diagnostics, Real-time PCR, COVID-19, Coronavirus, Infectious disease, Diagnostics
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