Evaluation of chemical compounds that inhibit neurite outgrowth using GFP-labeled iPSC-derived human neurons

•Neurite outgrowth is extensively used to evaluate compound with DNT potential.•Neurons labeled with GFP were derived from human iPSC.•Neurite outgrowth assay using GFP-labeled neurons was developed in a HCS platform.•Many selective neurite outgrowth inhibitors have been identified.•This HCS assay can be used to evaluate compound inhibition on neurite outgrowth.