Honing The Double-Edged Sword: Improving Human Ipsc-Microglia Models
FRONTIERS IN IMMUNOLOGY(2020)
Abstract
Human induced Pluripotent Stem Cell (hiPSC) models are a valuable new tool for research into neurodegenerative diseases. Neuroinflammation is now recognized as a key process in neurodegenerative disease and aging, and microglia are central players in this. A plethora of hiPSC-derived microglial models have been published recently to explore neuroinflammation, ranging from monoculture through to xenotransplantation. However, combining physiological relevance, reproducibility, and scalability into one model is still a challenge. We examine key features of the in vitro microglial environment, especially media composition, extracellular matrix, and co-culture, to identify areas for improvement in current hiPSC-microglia models.
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Key words
microglia, human, induced pluripotent stem cells, in vitro models, media composition, 3D scaffolds, co-culture, physiological relevance
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