Jinshanibacter, A New Genus Of Budviciaceae: Identification Of Jinshanibacter Zhutongyuii Sp. Nov. And Jinshanibacter Xujianqingii Sp. Nov. Isolated From Cloacal Content Of Snow Finch (Montifringilla Taczanowskii)

Four novel strains isolated from the cloacal contents of snow finches (Montifringilla taczanowskii) were characterized as aerobic, Gram-stain-negative, slightly motile, and rod-shaped. Analysis of the 16S rRNA gene sequence revealed that strain CF-458(T) had the highest similarities of 96.9 and 96.4 % with Limnobaculum parvum HYN0051(T) and Pragia fontium DSM 5563(T), while strain CF-1111(T) shared the highest similarities of 96.4 and 96.1% with Pantoea rodasii LMG 26273(T) and Pectobacterium punjabense SS95(T). Phylogenomic analysis showed the four isolates were separated into group. (CF-458(T) and CF-917) and group. (CF-1111(T) and CF-509), and clustered independently in the vicinity of the genera Limnobaculum and Pragia. Summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c, 23.9 and 17.2%, respectively), C-16:0 (21.8 and 22.1 %, respectively) and C-14:0 (10.6 and 17.7%, respectively) were the common major fatty acids, and summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c, 12.3%) was also a major fatty acid for strain CF-458(T) while cyclo-C-17:0 (13.1%) was for strain CF-1111(T). Both had Q-8 as the sole quinone and contained phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol as the major polar lipids. The DNA G+C content of strains CF-458(T) and CF-1111(T) was 45.7 and 45.4 mol%, respectively. Based on taxonomic position in the phylogenomic tree and phenotypic properties, two novel species of a new genus within the family Budviciaceae are thus proposed, with the name Jinshanibacter gen. nov., zhutongyuii sp. nov. (type strain CF-458(T)=CGMCC 1.16483(T)=GDMCC 1.1586(T)=JCM 33489(T)) and Jinshanibacter xujianqingii sp. nov. (type strain CF-1111(T)=CGMCC 1.16786(T)=GDMCC 1.1587(T)=JCM 33490(T)), respectively.