Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69-V70, France, August to December 2020.

Antonin Bal,Gregory Destras,Alexandre Gaymard,Karl Stefic,Julien Marlet,Sébastien Eymieux,Hadrien Regue,Quentin Semanas,Constance d'Aubarede,Geneviève Billaud, Frédéric Laurent,Claudia Gonzalez,Yahia Mekki,Martine Valette,Maude Bouscambert,Catherine Gaudy-Graffin,Bruno Lina,Florence Morfin,Laurence Josset, COVID-Diagnosis HCL Study Group

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin（2021）

Cited 191|Views13

No score

Abstract

We report the strategy leading to the first detection of variant of concern 202012/01 (VOC) in France (21 December 2020). First, the spike (S) deletion H69-V70 (ΔH69/ΔV70), identified in certain SARS-CoV-2 variants including VOC, is screened for. This deletion is associated with a S-gene target failure (SGTF) in the three-target RT-PCR assay (TaqPath kit). Subsequently, SGTF samples are whole genome sequenced. This approach revealed mutations co-occurring with ΔH69/ΔV70 including S:N501Y in the VOC.

Translated text

AI Read Science

Must-Reading Tree

Example

Generate MRT to find the research sequence of this paper

Chat Paper

Summary is being generated by the instructions you defined