Toxicity Of Bisphenol A On Marine Microalgae: Single- And Multispecies Bioassays Based On Equivalent Initial Cell Biovolume
SCIENCE OF THE TOTAL ENVIRONMENT(2021)
摘要
Standardised microalgal toxicity assays are usually carried out with single-species cultures; however, multispecies bioassays are more faithful to environmental conditions. The aim of this study was to assess the toxicity of the emerging pollutant bisphenol A (BPA) in single- and multispecies bioassays with three marine microalgae (Tetraselmis suecica, Phaeodactylum tricornutum and Nannochloropsis gaditana) using an equivalent initial cell biovolume of each species. After conducting preliminary growth experiments on these microalgae, a BPA concentration of 1 mg L-1 was chosen to study the effects of this pollutant in uni- and multialgal cultures. Growth, cell viability, oxidative stress, and inherent cell properties (size, complexity and autofluorescence) were determined by flow cytometry after 24 h of exposure. Results showed that P. tricornutum was the most affected species in all analysed biomarkers in both single- and multispecies bioassays. However, in N. gaditana cultures no significant differences were observed in any of the parameters and conditions tested, indicating that this species was the least sensitive to BPA. Between the uni- and multialgal tests, significant differences were observed in controls and BPA treated cultures of P. tricornutum and T. suecica. Nevertheless, it seemed that the cells of these marine microalgae suffered a similar pattern of alterations in both toxicity tests. (C) 2020 Elsevier B.V. All rights reserved.
更多查看译文
关键词
Bisphenols, Tetrasetmis suecica, Phaeodactylum tricornutum, Nannochtoropsis gaditana, Flow cytometry, Multialgal bioassays
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要