Blastocyst Complementation Reveals That Nkx2-1 Establishes The Proximal-Peripheral Boundary Of The Airway Epithelium

Background Distinct boundaries between the proximal conducting airways and more peripheral-bronchial regions of the lung are established early in foregut embryogenesis, demarcated in part by the distribution of SOX family and NKX2-1 transcription factors along the cephalo-caudal axis of the lung. We used blastocyst complementation to identify the role of NKX2-1 in the formation of the proximal-peripheral boundary of the airways in mouse chimeric embryos.Results While Nkx2-1(-/-) mouse embryos form primordial tracheal cysts, peripheral pulmonary structures are entirely lacking in Nkx2-1(-/-)mice. Complementation of Nkx2-1(-/-) embryos with NKX2-1-sufficient embryonic stem cells (ESCs) enabled the formation of all tissue components of the peripheral lung but did not enhance ESC colonization of the most proximal regions of the airways. In chimeric mice, a precise boundary was formed between NKX2-1-deficient basal cells co-expressing SOX2 and SOX9 in large airways and ESC-derived NKX2-1(+)SOX9(+) epithelial cells of smaller airways. NKX2-1-sufficient ESCs were able to selectively complement peripheral, rather than most proximal regions of the airways. ESC complementation did not prevent ectopic expression of SOX9 but restored beta-catenin signaling in Nkx2-1(-/-) basal cells of large airways.Conclusions NKX2-1 and beta-catenin function in an epithelial cell-autonomous manner to establish the proximal-peripheral boundary along developing airways.