Algorithms to Optimize Multi-Column Chromatographic Separations of Proteins.
JOURNAL OF CHROMATOGRAPHY A(2021)
摘要
The goal of this work was to provide a technical solution for the automated optimization of multi-column systems for protein separation and fractionation. Both algorithm and a software that can be downloaded are provided. In this algorithm, the length and order of the individual column segments can be considered. Various solutions are provided by the algorithm, including i) to obtain uniform peak distribution, ii) to park the different species at the inlet of the individual column segments, and iii) to elute all species as a single peak. Two representative examples are presented, showing the possibility to obtain uniform selectivity between monoclonal antibody (mAb) sub-units, and the on-column fractioning of intact mAbs. (C) 2020 The Author(s). Published by Elsevier B.V.
更多查看译文
关键词
Column coupling,On-column fractioning,Optimization,Multi-isocratic elution,Monoclonal antibody,Protein analysis
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要