Cytotoxicity Assessment of Antibiotics on Ctenopharyngodon Idellus Kidney Cells by a Sensitive Electrochemical Method.

As emerging pollutants, antibiotics are ubiquitous in the environment and pose a threat to human health, giving rise to an urgent need to assess their biological toxicity. In the present study, a cell electrochemical method based on the bromocresol violet/carbon nanotubes/glassy carbon electrode (BCP/MWCNTs/GCE) was established to evaluate the cytotoxicities of sulfamethoxazole (SMZ), ciprofloxacin (CIP), and tetracycline (TC). BCP/MWCNTs/GCE has advantages due to its excellent electrocatalytic activity for the oxidation of electroactive species of the Ctenopharyngodon idellus kidney (CIK) cells. The half-maximal inhibitory concentration (IC50) values of SMZ, CIP, and TC obtained by the electrochemical method were 831.51 μM, 354.98 μM, and 184.51 μM, which were lower than those of the traditional methyl-thiazolyl-tetrazolium (MTT) assay (907.47 μM, 414.87 μM, and 208.11 μM). These results indicate the higher sensitivity of the electrochemical method. This study provided a sensitive tool for the cytotoxicity evaluation of antibiotics in the environmental toxicology field.