An integrated quantitative proteomics strategy reveals the dual mechanisms of celastrol against acute inflammation

An integrated quantitative proteomics strategy to investigate the anti-inflammation effect of celastrol in a rat macrophage cell line against the LPS-induced acute response. The stable-isotopic based non-targeted quantitative profiling was performed to identify key regulatory proteins of celastrol response to LPS challenge, the dynamic changes of candidate proteins were then monitored by PRM quantitation and qPCR assay at corresponding time points to reveal the dual anti-inflammatory mechanisms of Celastrol against the LPS-induced acute inflammation.