Analysis of lipopolysaccharides by coupling microscale solid-phase extraction with capillary electrophoresis-laser induced fluorescence

•A novel portable microscale SPE device (μSPE) was developed for LPS extraction.•μSPE utilized small sample amount for sensitive and selective detection in CE-LIF.•μSPE was able to extract KP-LPS efficiently with high average recovery rate of 98%.•The enrichment of KP-LPS with μSPE was achieved by 23 times preconcentration.•μSPE was successfully applied on water samples with LPS concentration in ng mL−1.