Optimization of cultural conditions for hydrogen production by photosynthetic bacteria isolated from sewage water, Nalgonda, Telangana.

In this study, we have analyzed the hydrogen produced by purple photosynthetic bacterial consortium isolated from sewage water, Nalgonda. In presence of acetate, 6.0 ml of hydrogen was produced followed by benzoate and lactate as carbon sources. Lowest amount of hydrogen was produced in mannitol containing medium. In the presence of sodium nitrate and yeast extract as nitrogen sources more amounts of hydrogen was seen compared to other nitrogen sources. In Ammonium chloride and glutamate as nitrogen sources lowest amounts of hydrogen production was seen. In presence of cyanocobalamine as growth factor more amounts of hydrogen was produced. Riboflavin induced lowest amounts of hydrogen. T-Test was done on two samples assuming unequal variances and the results are presented.