Specific features of radical generation in the reaction of thiols with hydrogen peroxide

Using the method of inhibitors it was found that the reduction of H 2 O 2 with natural thiols in aqueous solutions is accompanied by the formation of radicals. The reaction of the nitroxyl radical TEMPO with glutathione (GSH) and H 2 O 2 was studied together and separately. It allowed us to determine the optimal conditions for using the spin trap method in the presence of thiols. In the case of 5,5′-dimethyl-1-pyrroline N -oxide the reaction of GSH and synthetic thiol N -acetylcysteine with hydrogen peroxide at pH < 7 leads to the formation of thiyl and hydroxyl radicals.