Assessment of Hemato-biochemical, mineral and oxidative changes in postpartum anoestrous (true anoestrus) Murrah Buffaloes

The present study was aimed to assess the hemato-biochemical, mineral and oxidative changes in postpartum anoestrous Murrah buffaloes. A total of 30 multiparous buffaloes of almost same age and body weight were used for the experiment. The experimental group I (GI) consisted of 15 postpartum anoestrous buffaloes (PPA) and control group II (GII) consisted of 15 normal cyclic buffaloes. Blood samples were collected by jugular vein puncture and analyzed for hematological parameters. Further, the serum was separated from another aliquot of blood sample and utilized for biochemical, mineral, MDA and SOD activity. The mean Hb, PCV, glucose, cholesterol and phosphorous values were significantly (p< 0.05) lower in PPA compared to cyclical buffaloes. The SOD activity and MDA concentration was significantly (p< 0.05) higher in PPA compared to cyclical buffaloes. In contrast, no significant (p>0.05) difference was observed in total protein and calcium concentration between groups. From the present study, it was concluded that postpartum anoestrous can be attributed to oxidative stress, which induces significant changes in hemato-biochemical and mineral profile.