Short Term Exposure Effects Of Trichostatin A

Histone deacetylase inhibitors (HDACi) are small molecules that increase acetylation of lysine residues by ultimately inhibiting the activity of histone deactylase enzymes. These anticancer agents affect epigenetic and non-epigenetic regulation resulting in cell cycle arrest of cancer cell lines. We have conducted high throughput screen to elucidate effect of short term exposure to Trichostatin A on transcriptional activity of a model cell line. We have tested short term exposure (2h) of Trichostatin A (10uM) on ARPE-19 cell line.