What Is The Correct Stoichiometry Of Kv2.1:Kv6.4 Heteromers?

Moller et al. (1) propose that the predominant native stoichiometry of Kv2.1:Kv6.4 heteromeric K+ channels is 2:2, a finding that is unexpected based on past analyses which support a 3:1 stoichiometry for Kv2.1:KvS regulatory subunit heteromers, including Kv6.4 and Kv9.3 (2⇓-4). Like Moller et al. (1), we used a combination of total internal reflection fluorescence (TIRF) microscopy and electrophysiology to examine the native stoichiometry of Kv2.1:Kv6.4 heterotetramers (5). However, we reached the conclusion that 3:1 heteromers are the predominant functional species for these heteromers. We suspect the differences in our findings result primarily from the TIRF data collection strategies. We note that both studies find support for … \n\n[↵][1]1To whom correspondence may be addressed. Email: tjj3{at}psu.edu.\n\n [1]: #xref-corresp-1-1