基于发酵床养猪垫料的地衣芽胞杆菌FJAT-4固态发酵培养条件的优化
Journal of Biological Control(2017)
Abstract
为提高枯萎病生防菌地衣芽胞杆菌FJAT-4的固态发酵水平,以农业副产物——微生物发酵床养猪垫料为主要培养基成分,以活菌体数量为指标,通过单因子试验对菌株FJAT-4的最适发酵培养基成分及发酵条件进行优化.结果表明,菌株FJAT-4最适发酵培养基配方为垫料:麸皮(质量比)=7:3、K2HP040.05%、MgS04 0.025%;最佳发酵培养条件为温度35℃,相对湿度45%,通气量75%,发酵周期40 h.在最佳发酵培养基和发酵条件下,菌株FJAT-4固态发酵物干重活菌体数可达2.63×109 cfu/g.
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Microbial Metabolism
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