Study Of Let 7 Mirnas In Cumulus Cells Of Women Undergoing Ivf.

To investigate the expression of let7b and let 7c genes in women undergoing IVF This is a single-centre study carried out in the molecular biology laboratory of the IVF unit of a gynecological and obstetrics department spanning the period September 2018 to September 2019. We studied the expression of let 7b and c genes in cumulus cells of 25 women undergoing IVF treatment RNA was extracted from cumulus cells of 25 women using Monarch total RNA kit. Subsequently cDNA was synthesized by using Takara primescipt cDNA synthesis kit and Real time PCR (LightCycler480II) using SYBR Green and specific primers for let 7b and c genes was applied. The expression of let 7b was higher (mean value of Cp 28.7) than the expression of let7c (mean value of Cp 36.2), indicating that the let 7b gene may be a biomarker, associated with the maturation and function of cumulus cells. High expression levels of various let-7 family members have been reported in cumulus cells. Additionally, let-7 miRNAs are negatively associated with pluripotency although they are highly expressed in oocytes indicating a high degree of tissue specificity of miRNA expression. Our findings are in accordance with previous results and underline the likelihood that they are involved in regulating different processes at specific time points. Our findings show that the let7b gene has a higher expression than the let7c gene in cumulus cells indicating a possible mechanism involving miRNAs in female infertility. The limitations of our study include our small group size. Larger sample would be valuable and potentially helpful in critically evaluating our findings. Detecting candidate genes associated with overexpressed or downregulated miRNAs such as let 7b appears essential, for the better understanding of the mechanism underlying the oocyte-cumulus cells complex