Dosage and evaluation of the antiradical activity of alkaloids of the root barks of Oulotricha Le Thomas: isolation of Liriodenine

Oulotricha Le Thomas, a subspecies of Annona senegalensis (Annonaceae), is a plant present in all the Congolese savannahs whose mature fruits are widely consumed by the said population and sometimes edible. It is used traditionally to treat infections and some illness. Today, there are more than 160 alkaloids characterized in Annonaceae but any isoquinolin are not isolated of this plant. As a result, an extraction of the alkaloids confirmed by TLC after revelation with KI-Iodine, followed by a spectral scanning assay these alkaloids were made. Subsequently, this extract was fractionated until the isolation one compound and evaluated for anti-radical activity. On an extraction yield of 0.3% of the alkaloids was obtained 0.02% of isolated Liriodenine. The dosage of alkaloids at wavelengths between 254.5 and 360.5 nm detected three peaks of characteristic absorbance whose the maximum absorbance was observed at 320 nm. This extract exhibited a good anti-radical activity.