Influence of LFS on Inducing Sugarcane Callus

Objective: To establish the technology for inducing efficiently the sugarcane callus with high quality.Methods: Take young leaf cuts of sugarcane as explants,and MS as basic medium added respectively different concentrations of new inductive agent LFS,and with the traditional inductive agent 2,4-D as ck for contrast research. Results: MS + LFS 0. 05- 1. 00 mg / L could all induce callus with high efficient and quality. The suitable concentration of LFS was 0. 20 mg / L,cultivated 20 d,callus induction rate reached 98%,browning and hardening slightly,greenish rate reached 16. 8%. Such callus was very suitable for organ differentiation in further. Conclusion: LFS is better than 2,4-D to induce efficiently sugarcane callus with high quality and simplifing significantly the productive process.